Abstract
Ovary-secreted autocrine/paracrine factors play important roles in regulating oocyte maturation via the autocrine/paracrine pathway. This study aimed to evaluate the functions of bone morphogenetic protein 2 (BMP2) in oocyte maturation and communication between follicle cells and oocytes. In our study, we first identified BMP2 from the mud crab Scylla paramamosain. Quantitative real-time PCR showed that BMP2 was detected in diverse tissues, notably in the ovary, stomach and gill. The expression levels of BMP2 transcripts increased during vitellogenesis. Spatial expression of BMP2 and receptors in the ovary revealed that BMP2 was exclusively detected in oocytes, whereas the receptors were expressed in both follicle cells and oocytes. RNAi tests revealed that the expression of cyclin B first decreased at 2 h and then increased at 4 h after BMP2 knockdown. These combined findings suggest that BMP2 may promote oocyte maturation through an autocrine/paracrine pathway in S. paramamosain.
Highlights
In vertebrates, oocyte maturation is a complicated mutual interaction between extraovarian and intraovarian signals (Ge, 2005; Emori and Sugiura, 2014)
We reveal the function of bone morphogenetic protein 2 (BMP2) in oocyte maturation via an autocrine/paracrine pathway in S. paramamosain
We first designed specific primers to amplify the sequences of BMP2 and the green fluorescent protein (GFP) gene (Table 1)
Summary
Oocyte maturation is a complicated mutual interaction between extraovarian and intraovarian signals (Ge, 2005; Emori and Sugiura, 2014). Oocyte maturation is generally activated by luteinizing hormone (LH), which is produced and secreted from the pituitary (Nagahama and Yamashita, 2008). In addition to this classical endocrine pathway, growing evidence suggests that many intraovarian autocrine/paracrine factors, such as transforming growth factor β (TGFβ) and epidermal growth factor (EGF), play critical roles in oocyte maturation via the autocrine/paracrine pathway (Ge, 2005; Clelland and Peng, 2009). Rat BMP9 plays an autocrine role in regulating steroidogenesis in ovarian granulosa cells (Hosoya et al, 2015). BMP2 stimulates steroidogenesis in granulosa cells through an autocrine pathway (Inagaki et al, 2009)
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