Abstract
The fetal liver (FL) has been proposed a source of hematopoietic stem and progenitor cells (HSPCs) for transplantation therapy. However fetal HSPCs at distinct developmental stages reconstitute similarly or differently in recipient bone marrow (BM) remains undetermined. We tested this in a syngeneic mouse transplantation model. We first analyzed lineage-components of FL from 12.5 to 18.5 days postfertilization (dpf). The myeloid (GM) and lymphoid (B and T) were dynamically changed at different trends with biggest difference in between 12.5 and 16.5dpf. The HSPCs (Lin—CD150+CD48—) at 12.5 and 16.5dpf were then respectively transplanted into recipient mice. The difference was significantly reduced at 4wk and undetectable afterward; the BM environment assimilated them. Profiling lineage-specific genes’ expression in the HSPCs of input and outcome in the recipients showed the expressions became almost the same in the engrafted HSPCs. Together the recipient BM microenvironment determined the developmental lineage-trends of FL-HSPCs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
