Abstract
BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-tration of metal ions (0.2 mM to 1.4 mM), pH (3 to 8) and temperature of incubation (15℃, 20℃, 25℃, 30℃ and 35℃). Results of BOD inhibition are supported by absorbance measurement (OD600) studies of microbial matter preserved in Luriya broth medium. OD measurement results are used to derive minimum inhibitory concentration, i.e., threshold concentration of each metal showing toxicity towards microbes. Sil-ver is found to be the most toxic element.
Highlights
Results of BOD inhibition are supported by absorbance measurement (OD600) studies of microbial matter preserved in Luriya broth medium
In the normal BOD measurement process, microbial matter is taken from some source that is known to be rich in bacteria and capable of undergoing BOD exertion process
It has been established that the type of inoculum becomes more important [1,2] for a study where BOD exertion is measured at different concentrations of the heavy metal ions
Summary
In the normal BOD measurement process, microbial matter is taken from some source that is known to be rich in bacteria and capable of undergoing BOD exertion process. Through one of the probable mechanisms, heavy metals are transported across the cell membrane of the microbe and tend to impair the biochemical process responsible for the exertion of BOD These metals are generally transition elements and have unfilled d-orbitals, which help the metal ions to complex with chelating molecules like enzymes proteins and amino acids. Effect of metals like cobalt, nickel, copper, zinc, silver and cadmium on BOD3 has been studied using wastewater from dairy industry as a seed. They were selected for experimentation because of their widespread industrial use and known toxicity to aquatic organisms. Spectrophotometer: Spectrophotometer (U2800, Hitachi) has been used for measurement of optical density at 600 nm
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