Abstract
Objective To construct eukaryotic expression vector of human bon e morphogenetic protein 2, verify its expression in transfected human mesenchymal stem cells (hMSCs) and its effect on hMSCs differentiation. Method The BMP2 gene was cloned into a eukaryotic expression vector pcDNA3. The recombinant was tran sfected into hMSCs by liposome. Immunnohistochemistry and in situ hybridization methods were used to identify the expression of BMP2 mRNA and protein; ALP and V on Kossa stains were performed to identify the differentiated effect on the hMSC s. Result The pcDNA3/BMP2 fragments were the same as the theory has stated. BMP2 were expressed and synthesized both in 48h and 4w, and the osteoblastic differe ntiation was promoted. Conclusion Transfection of pcDNA3/BMP2 can provide transi ent and persistent expression in hMSCs, and promote the MSCs differentiation.
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