Abstract
Application of minigels and the PhastSystem™ to obtain phenotyping results from bloodstains in the EAP, Hp, AK, and Glo I typing systems was investigated. Nonequilibrium isoelectric focusing with 4–6.5 PhastGel™ produced readily interpretable phenotypes in the EAP typing system. Both 4–6.5 and 5–8 PhastGel™ produced AK typing system phenotypes using nonequilibrium isoelectric focusing conditions. The 8–25% PAG PhastGel™ developed by two staining techniques allowed discrimination of phenotypes for the Hp typing system. Phenotypes from the Glo I typing system were also obtained with this gel type. Variant haemoglobins could be detected on pH 5–8 PhastGel™ using isoelectric focusing conditions. Much potential for standardized, rapid phenotyping of bloodstains was found to exist utilizing the Phastsystem™.
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