Blood to cerebrospinal fluid barrier in spontaneous hypertension and ventricular dilation

Abstract

Background Arterial hypertension produces ventricular dilation, CSF protein composition variations as well as alterations in the circumventricular organ and choroid plexus (CP). Cerebrospinal fluid (CSF) has an important relationship with the blood and the brain, and encephalic pathology could alter the connections between the blood, brain and CSF, and consequently the protein composition of the CSF. Furthermore, certain proteins could be used as markers such as transthyretin and S-100β, which are extravasated when central nervous system (CNS) barriers are breached. The S-100β is primarily synthesized in the brain by the astrocytes and is released into the blood when the blood brain barrier (BBB) is disrupted. Transthyretin is a protein primarily located in the brain in choroid plexus (CP), subcommissural organ (SCO) and, as soluble monomer, in the CSF, where it has a positive gradient for extravasation in peripheral blood, when the blood to CSF barrier (BCB) is altered. The aim of the present work is to analyze the CNS barriers in the arterial hypertension using antibodies against S-100β, transthyretin monomer (TTRm), TA-p73 and Reissner fibre (AFRU). Materials and methods Brain, cerebrospinal fluid and serum were extracted from control Wistar-Kioto (WKY) rats and spontaneously hypertensive rats (SHR) of 10 months of age. Brain sections containing the SCO and the CP were processed by immunohistochemistry using the following as primary antibodies: anti-S-100β, anti-TTRm anti-TA-p73 and AFRU. Western blot and 2-D electrophoresis of CSF, serum and extracts of SCO and CP were performed.