Abstract

1. 1. Theoretical models can fit the oxygen equilibria of trout and human red cell suspensions, and describe the apparent oxygenation process in the red cell. 2. 2. The assumption that full oxygenation is attained at atmospheric O 2 pressures can result in biphasic Hill plots and high Hill coefficients for high O 2 saturations. This phenomenon must not be confused with aggregation of hemoglobin. 3. 3. Problems specific to measurements of red cell suspensions, regarding the ionic cellular composition and its stability with time, are approached. Changes of buffer osmolarity, and—for trout—addition of adrenaline, within physiological proportions, have no impact on the results. 4. 4. This tends to validate the general significance of equilibrium data obtained on this material, regarding the effects of protons and organic phosphates, although complex ionic movements across the red cell membrane are known to occur in the animal under certain circumstances.

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