Block of proliferation 1 overexpression in hepatocellular carcinoma and its promoting effect on migration of hepatocellular carcinoma HepG2 cells

Abstract

Objective To study the expression of block of proliferation 1 (BOP1) gene in hepatocellularcarcinoma (HCC) and its impact on the proliferation andmigration of HepG2 cell line.Methods Real-time fluorogenic quantitative polymerase chain reacton (RFQ-RCR) was conducted to quantify the expression of BOP1 mRNA in the normal and cancerous tisseus from 45 patients with HCC.The eukaryotic vector of human BOP1 was constructed,and transfected into HepG2 cells.The cell counting kit-8 (CCK-8) assay and Scratch test were used to oberserve the influence of BOP1 overexpression on proliferation and migration of HepG2 cells respectively.Results In 45 patients with HCC,the global expression (△Ct) of BOP1 was (15.16 ± 1.86) in tumor tissue and (17.48 ±2.68) in corresponding normal tissue respectively.BOP1 mRNA expression in tumor tissues was significantly higher than in the normal tissues (P ＜0.01).The results of CCK-8 assay showed that the A value in experimental group was higher than in control group,and overexpression of BOP1 significantly promoted cell proliferation (P ＜ 0.05).Scratch text revealed that the speed of scratch healing in experimental group was significantly faster than in control group (P ＜ 0.05).Seventy-two h after transfection,the control group still had a fissure,but the experimental group had completely healed.Overexpression of BOP1 could obviously enhance the migration of HepG2 cells.Conclusion BOP1 gene has a higher expression level in HCC,and overexpression of BOP1 can increase proliferation and migration of HepG2 cells.This gene probably playsan important role in carcinogenesis of HCC. Key words: Carcinoma,hepatocellular; Block of proliferation 1; Gene expression