Abstract
In this study, decolorization of the diazo dye, Reactive Black 5 (RB5) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experiments were conducted at different RB5 and hydrogen peroxide concentrations in a batch manner to estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. An acceptable agreement was observed between the model and experimental data.
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