Abstract
Fusarium moniliforme, a fungus that commonly produces a symptomless endophytic association with most corn cultivars and inbred lines, was previously shown to catabolize 6-methoxy-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), biologically active compounds produced by corn and known to be fungistatic. Studies were undertaken to isolate and identify the breakdown products of this decomposition. For each benzoxazolinone, a novel acid was isolated by column chromatography from 48-h old cultures of F. moniliforme. MS, NMR, and IR spectral data were used to identify the compounds as N-(2-hydroxyphenyl) and N-(2-hydroxy-4-methoxyphenyl) malonamic acids, products of BOA and MBOA, respectively.
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