Abstract
Guinea pig liver microsomes were tested for their capability to catalyze the incorporation of spin-labelled stearic acid into phospholipids. A system utilizing sn-glycero-3-phosphate-2- 14C, 12-doxyl-stearic acid, and microsomal fractions isolated from guinea pig liver yielded a phospholipid, identified as phosphatidic acid containing 14C and spin label. These experiments established the capability of a mammalian enzyme system to utilize spin-labelled stearic acid in the biosynthesis of a phospholipid.
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More From: Biochemical and Biophysical Research Communications
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