Abstract
Objective: The present study was aimed to investigate gold nanoparticles synthesized by fungal isolate Neosartorya udagawae and determination of their stability in biofluids to probe their aptness in drug delivery applications.Methods: In this procedure, gold nanoparticles were prepared by biosynthesis using seven days old culture of Neosartorya udagawae and aqueous chloroauric acid. After the complete reaction, the fungal biomass was subjected to UV-Vis, XRD, FT-IR Spectrum analysis, TEM, Zeta potential, SEM and EDX analysis.Results: Intra/extracellular synthesis of gold nanoparticles was confirmed by a sharp peak at 526 nm in UV spectroscopy. SEM, TEM analysis demonstrates the spherical shape of AuNPs with an average diameter of 50 nm and XRD confirm the crystalline gold nanoparticles. FTIR analysis reveals the presence of the protein shell around the gold nanoparticles. The zeta potential value of AuNPs was-36mV which confirmed the stability of nanoparticles dispersion. Gold nanoparticles have shown high stability in biofluids of Bovine Serum Albumin and Phosphate Buffer Saline at pH-5, pH-7and pH-9 which mimic the human colonic biological environment.Conclusion: The fungal synthesis of AuNPs has been experimentally demonstrated and their stability in BSA, 10% NaCl and PBS at pH-7. This might be a promising option for drug delivery applications in carcinogenic colon disorders in human beings.
Highlights
Biological synthesis of nanoparticles is unloading amplified attention in the recent past by the emerging intersection between nanotechnology and biotechnology
Fungal biomass changed from yellow to light pink on 12h incubation as shown in fig. 1c is in agreement with the extra-/intracellular biosynthesis of gold nanoparticles by the fungus Penicillium Sp. [15]
90-95% of conversion of gold ions to nanoparticles was achieved within 24 h through a complete change to dark purple color of biomass which is a visual indication of intracellular accumulation of gold nanoparticles
Summary
Biological synthesis of nanoparticles is unloading amplified attention in the recent past by the emerging intersection between nanotechnology and biotechnology. Nano-biotechnology is an imminent field in the nanoscience It is the control of biological material to yield nanocomposites of various sizes and shapes. Researchers focused their interest on non-toxic and environmentally friendly nanoparticles by microorganisms like fungi, [4] bacteria, [5] and algae [6] for the synthesis of nanoparticles which offers numerous benefits with compatibility in biomedical applications. The intracellular synthesis of nanoparticles is a two-step process, the first is trapping metal ions on the fungal cell surface through electrostatic interaction of positively charged groups in enzymes present in the cell wall mycelia, secondly to metal ions reduced within the cell wall which increase the synthesis of nanoparticles [7]. Epicoccum nigrum fungi isolated from gold mine soil in Iran [9] and alkalotolerant actinomycetes Rhodococcus species [10] were good biological tools for AuNPs synthesis. Biosynthesised AuNPs were characterized by UV-Spec, SEM, TEM, and zeta potential, EDX, XRD and FT-IR
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