Biosynthesis of ethylene in higher plants: the metabolic site of inhibition by phosphate*

Abstract

Abstract. Phosphate inhibited endogenous as well as 1‐aminocyclopropane‐1‐carboxylic acid (ACC)‐stimulated ethylene synthesis in slices of tomato fruit, segments of carrot root and pea hypocotyls. ACC concentrations of up to 10 mol m−3 did not overcome this inhibition. Phosphate inhibited the conversion of 14C ACC to ethylene in tomato fruit and vegetative tissue. Enzymatic conversion of ACC to ethylene by pea seedling homogenate was also inhibited by phosphate with a linear concentration dependency. The formation of ACC from S‐adenosylmethionine (SAM) by extracts of pink tomatd fruit was slightly, but not significantly, affected by phosphate. However, the SAM to ACC conversion was greater when extracts from tomato fruit were made in phosphate rather than in HEPES‐KOH buffer. Non‐enzymatic ethylene synthesis from ACC in a model system was stimulated by phosphate. We suggest that phosphate is an inhibitor of ethylene biosynthesis in higher plants and that one site of its control is the conversion of ACC to ethylene.