Abstract
Phosphate-solubilizing bacteria isolated from the rhizosphere of wheat and rye plants produced plant growth regulating substances of the auxin type when cultivated in liquid culture supplemented with tryptophane. The amount of auxins found in culture filtrates varies from 0.01 to 3.98 mg indole-3-acetic acid (IAA) equivalent per litre culture medium. Significant differences were noted in the amount of auxins produced by different strains. A bioassay, thin layer chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry were used for characterization of the auxins. Indoleacetic acid was identified in culture filtrates of 4 Pseudomonas and 1 Acinetobacter strains.
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