Abstract
Chlorophyll formation in etiolated barley leaves is inhibited by treatment with 500 μm-3-amino-2,3-dihydrobenzoic acid (gabaculine). Significant amounts of glutamate 1-semialdehyde and small amounts of δ-aminolevulinate accumulated in the treated leaves. Aerobic conditions and light stimulated the accumulation of glutamate 1-semialdehyde. Gabaculine (5 μm) inhibited the activity of purified glutamate 1-semialdehyde aminotransferase by 83%. This inhibition was irreversible and required the presence of either glutamate 1-semialdehyde or δ-aminolevulinate. The mass and infrared spectra of glutamate 1-semialdehyde formed in gabaculine treated leaves were identical to the spectra of the chemically synthesised compound. The experiments confirm that glutamate 1-semialdehyde participates as an intermediate in chlorophyll biosynthesis. Glutamate 1-semialdehyde hydrochloride was stable.
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