Abstract
Bioconversion of agro-waste biomass into ethanol through cellulase-producing microbes has been a trending research area in recent years. The present work focused on the isolation, characterization, and optimization of potent cellulase enzyme-producing bacterial isolate from Bandipur forest soil. Among the three bacterial isolates, one isolate exhibited the highest cellulase enzyme activity of 147.23 U/ml at pH 7 and a temperature of 30 °C with an incubation time of 72 h. Based on molecular taxonomical studies, the isolate was identified as Chryseobacterium culicis strain Bp16. Further, under optimal conditions, a minimal medium supplemented with various biomasses like coconut husk, Pongamia leaves, rice bran, and wood waste survived as a source for bacterial cellulase enzymatic activity and produce reducing sugars under submerged fermentation. Further investigation revealed that the fermentation of reducing sugars in a coconut husk and rice bran as substrates resulted in 0.53% ethanol production via the action of baker's yeast and wine starter cultures respectively. The outcome of the present research allowed us to explore a potent and novel cellulolytic bacterium, Chryseobacterium culicis strain Bp16 for ethanol production through bioprocessing of waste biomass.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
