STAR Protocols | VOL. 3

Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking

Publication Date Jun 1, 2022


Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique. For complete details on the use and execution of this protocol, please refer to Lee etal. (2021).


Bioprinting System Continuous Printing Tumor Microenvironment Multilayered Constructs Continuous Technique Continuous System Physiological Functions Advanced Technique Cell-hydrogel Constructs Biofabrication Technique

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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