Biomarker profiling in osteoarthritis: Hematological and inflammatory insights from a cross-sectional study

The aim of the present study was to investigate the relationships between red blood cell distribution width, platelet distribution width, and mean platelet volume and the presence and severity of valvular involvement in patients with rheumatic heart disease. Between April, 2012 and December, 2015, 151 patients who were admitted to the Pediatric Cardiology Unit with diagnosis of rheumatic heart disease and 148 healthy children were included to our study. Transthoracic echocardiography for all children was performed, and the values of red blood cell distribution width, platelet distribution width, and mean platelet volume, besides other blood count parameters, erythrocyte sedimentation rate, and C-reactive protein levels were recorded. Red blood cell distribution width, platelet distribution width, mean platelet volume, and C-reactive protein levels were significantly higher in patients with rheumatic heart disease when compared with healthy controls (p0.05). This is the first study in children with rheumatic heart disease that demonstrated significantly increased red blood cell distribution width, platelet distribution width, and mean platelet volume levels, as well as evaluated all three parameters together. Furthermore, red blood cell distribution width values in the chronical period of acute rheumatic fever, due to the positive correlation with the other chronic inflammatory markers, may help make the diagnosis in children.

The prevalence of thyroid carcinoma is increasing, and papillary thyroid carcinoma (PTC) is the most frequent subtype. More and more attention is being concentrated on the association between inflammation indicators and malignant tumors. The aim of the present study was to analyze whether the preoperative red blood cell distribution width (RDW) and platelet parameters, including mean platelet volume (MPV) and platelet distribution width (PDW), can be applied to distinguish between patients with PTC or papillary thyroid microcarcinoma (PTMC) and healthy controls, and to explore the associations with clinicopathological characteristics. The study retrospectively compared the RDW, MPV and PDW values of 780 patients with PTC or PTMC against a healthy control group. Receiver operating characteristic (ROC) curves were conducted to determine diagnostic accuracy. Furthermore, the clinicopathological features of the patients with PTC or PTMC were compared between higher and lower platelet parameter groups based on the RDW, MPV and PDW values. Significantly higher preoperative RDW, MPV and PDW values were found in patients with PTC or PTMC compared with those of the healthy group. ROC curve analysis showed that the area under the curve (AUC) plus 95% confidence interval (95% CI) values of RDW, MPV and PDW were 0.808 (0.780-0.835), 0.771 (0.743-0.799) and 0.711 (0.681-0.742), respectively. When RDW and MPV were combined together, the AUC (95% CI) value was enhanced to 0.858 (0.835-0.881) for the patients with PTC. For the patients with PTMC, RDW, MPV and PDW had AUC (95% CI) values of 0.812 (0.783-0.840), 0.779 (0.749-0.808) and 0.718 (0.685-0.751), respectively. When RDW and MPV were combined together, the AUC (95% CI) value was enhanced to 0.858 (0.835-0.881). A higher RDW was significantly associated with being female, deeper tumor infiltration, and normal FT3 and FT4 levels. A higher PDW was significantly associated with elevated thyrotropin receptor antibody levels. In conclusion, as convenient and available inflammation indicators, RDW, PDW and MPV have diagnostic ability and can distinguish between patients with PTC or PTMC and healthy controls. In addition, the combined application of RDW and MPV can improve the diagnostic power. The values of RDW and MPV were associated with clinicopathological characteristics. To the best of our knowledge, this is the first study to prove the usefulness of preoperative RDW combined with MPV in diagnosing patients with PTC or PTMC.

Theileria parasitises red and white blood cells.T. annulata causes bovine tropical theileriosis; T. parva causes East Coast fever; and T. orientalis causes oriental theileriosis.In tropical regions, oriental theileriosis is a serious livestock illness mainly affecting red blood cells (RBCs).This study aimed to ascertain the prevalence of theileriosis infection in animals kept at the KVASU farms that appeared to be in good health.Additionally, the study sought to explore the possible correlation between the infection of Theileria and haematological markers in crossbred and Vechur cattle.The haematological parameters and Theileria infection status of fifty adult cattle were assessed.Monocytes, PDW (platelet distribution width), RDW (RBC distribution width), MPV (mean platelet volume), MCHC, HCT, RBC, HGB, and MCV demonstrated a significant breed difference (P<0.05).The RBC, WBC, MCV, MCHC, MCH, MPV, PCT, and PDW levels differed significantly (p<0.05) between animals infected with Theileria and the uninfected controls.For RBC, WBC, HGB, MCV, MCH, MPV, and PDW, there was a significant interaction (P<0.05) between the breed and Theileria infection.RBC, MPV, and PDW significantly increased in Theileria-infected crossbred cattle.On the other hand, RBC, MCV, MCH, MPV, and PDW significantly increased (P<0.05) in infected Vechur cattle.The study's results suggest that various haematologic indicators could be used as diagnostic biomarkers for Theileria infection in crossbred and Vechur cattle.

The objectives of the study were to determine differences in the parameters of red blood cells (RBC), white blood cells (WBC), and platelets at low altitude (LA) and at high altitude (HA) and with the gestation being advanced, and to determine correlations between parameters of RBC and platelets. We also studied the association of RBC and platelets with markers of iron status. In addition, markers of iron status and inflammation were measured and compared at each trimester of gestation in pregnant women at LA and HA. A cross-sectional comparative study was conducted at Lima (150 m above sea level) and Cusco at 3400 m above sea level from May to December 2019. Hematological parameters in pregnant women (233 at LA and 211 at HA) were analyzed using an automated hematology analyzer. Serum ferritin levels, soluble transferrin receptor (sTfR), hepcidin, erythropoietin, testosterone, estradiol, and interleukin-6 (IL6) levels were measured by ELISA. One-way ANOVA supplemented with post hoc test, chi-square test, and Pearson correlation test statistical analyses were performed. p < 0.05 was considered significant. Pregnant woman at HA compared to LA had significantly lower WBC (p < 0.01), associated with higher parameters of the RBC, except for the mean corpuscular volume (MCV) that was no different (p > 0.05). Platelets and mean platelet volume (MPV) were higher (p < 0.01), and platelet distribution width (PDW) was lower at HA than at LA (p < 0.01). A higher value of serum ferritin (p < 0.01), testosterone (p < 0.05), and hepcidin (p < 0.01) was observed at HA, while the concentration of sTfR was lower at HA than at LA (p < 0.01). At LA, neutrophils increased in the third trimester (p < 0.05). RBC parameters decreased with the progress of the gestation, except RDW-CV, which increased. The platelet count decreased and the MPV and PDW were significantly higher in the third trimester. Serum ferritin, hepcidin, and serum testosterone decreased, while sTfR and serum estradiol increased during gestation. At HA, the WBC and red blood cell distribution width- coefficient of variation (RDW-CV), PCT, and serum IL-6 did not change with gestational trimesters. RBC, hemoglobin (Hb), hematocrit (Hct), mean corpuscular hemoglobin concentration (MCHC), and platelet count were lower as gestation advanced. MCV, MPV, and PDW increased in the third trimester. Serum ferritin, testosterone, and hepcidin were lower in the third trimester. Serum estradiol, erythropoietin, and sTfR increased as gestation progressed. Direct or inverse correlations were observed between RBC and platelet parameters and LA and HA. A better number of significant correlations were observed at HA. Hb, Hct, and RDW-CV showed a significant correlation with serum ferritin at LA and HA. Of these parameters, RDW-CV and PDW showed an inversely significant association with ferritin (p < 0.05). In conclusion, a different pattern was observed in hematological markers as well as in iron status markers between pregnant women at LA and HA. In pregnant women a significant correlation between several RBC parameters with platelet marker parameters was also observed. Data suggest that pregnant women at HA have adequate iron status during pregnancy as reflected by higher serum ferritin levels, lower sTfR levels, and higher hepcidin values than pregnant women at LA.

Sickle cell anaemia is one of the life threatening health challenges bedevilling many developing countries like Nigeria and the management requires regular monitoring of the patients. The aim of this study was to determine haematological parameters and inflammatory markers among paediatrics with sickle cell anaemia. A total of 90 subjects were recruited for this study comprising of 45 confirmed sickle cell anaemia patients in steady state, 32 males and 13 females and 45 apparently healthy subjects with haemoglobin genotypes AA were used as the controls. Automated blood analyzer was used to analyze haematological parameters, erythrocyte sedimentation rate was estimated by Westergreen method and C-reactive protein was estimated using Enzyme Linked Immuno-Sorbent method. Total white blood cell count, white cell differential count, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, red cell distribution width, platelet count, platelet distribution width, erythrocyte sedimentation rate, C-reactive protein was higher in case compared to control. However, red blood cell count, haemoglobin, haematocrit, plateletcrit and mean platelet volume were lower in the case compared to the control subjects. There is no significant (p&gt;0.05) difference in haematological parameters, erythrocyte sedimentation rate and C-reactive protein among the gender in both the case and the control except plateletcrit in the case and eosinophil and red cell distribution width in the control. There is similarity in haematocrit in both sex among the case. It is our anticipation that these findings will greatly assist physicians in the better management of Sickle cell patients in our locality.

We aimed to determine the relationship of red cell and platelet distribution widths with the onset of acute myocardial infarction, to enable the early detection and prevention of acute myocardial infarction. Red cell and platelet distribution widths were retrospectively determined in 46 patients with stable angina pectoris and 140 patients with acute myocardial infarction who were brought to the emergency department of our institution. Red cell and platelet distribution widths were determined with an automatic blood cell analyzer, and the results were compared between the acute myocardial infarction and angina pectoris groups. Both red cell and platelet distribution width values obtained at onset were significantly higher in the acute myocardial infarction group than in the angina pectoris group (red cell distribution widths, 46.4 ± 0.51% versus 44.5 ± 0.59%; mean difference -1.91 [95% confidence interval (CI), -3.79 to -0.34]; platelet distribution widths, 12.1 ± 0.22 fL versus 11.1 ± 0.17 fL; mean difference -1.03 [95% CI, -1.58 to -0.47]). The red cell distribution widths before onset was not different between the acute myocardial infarction and angina pectoris groups; however, the platelet distribution widths before onset was higher in the acute myocardial infarction group (red cell distribution widths, 46.5 ± 0.85% versus 45.9 ± 0.59%; mean difference -0.71 [95% CI, -2.74 to 1.30]; platelet distribution widths, 11.4 ± 0.39 fL versus 10.6 ± 0.21 fL; mean difference -0.83 [95% CI, -1.66 to 0.11]). Red cell distribution widths and especially platelet distribution widths may contribute to the early detection of acute myocardial infarction.

Introduction: Urinary Tract Infections (UTIs) are a prevalent global health issue, primarily caused by bacterial infections. Early diagnosis and intervention are critical to preventing complications like pyelonephritis and mitigating the risk of recurrent infections that can lead to permanent scarring. Aim: To investigate the haematological parameters in patients with confirmed UTIs, comparing them with non UTI counterparts and discerning variations in haematological parameters between UTIs caused by gram-positive and gram-negative bacterial organisms. Materials and Methods: This prospective case-control study was conducted at Chettinad Hospital and Research Institute (tertiary care centre), Chengalpattu, Tamil Nadu, India between January 2023 and July 2023, included 105 culture-positive UTI cases and 105 age- and gender-matched culture-negative controls. Cases included patients over 12 years of age with confirmed UTIs, while exclusions for both groups included pregnancy, individuals under 12 and those with haematological disorders. Controls were also excluded if they had diabetes or chronic kidney disease. Urine cultures on blood and MacConkey agar identified pathogens such as E. coli, Pseudomonas, Klebsiella, Enterobacter, Proteus and Citrobacter. Grampositive isolates included Enterococci species, Staphylococcus aureus and Staphylococcus saprophyticus. Haematological parameters such as Haemoglobin (Hb), White Blood Cell Count (WBC), platelet count, Mean Platelet Volume (MPV), Red Cell Distribution Width (RDW), Platelet Distribution Width (PDW) and Plateletcrit (PCT) were analysed using an automated haematology analyser. Statistical analysis was performed using Statistical Package for Social Sciences (SPSS) software version 22.0 with significance set at p-value &lt;0.05. Results: The UTI patients exhibited lower Hb and higher WBC, platelet count, MPV, RDW, PDW and PCT compared to controls. Gram-positive UTI infections showed higher WBC count, platelet counts, RDW, PDW and PCT, but lower MPV compared to gram-negative UTI infections. E. coli and Enterococcus were the predominant gram-negative and gram-positive pathogens, respectively, reflecting distinct haematological profiles. These results highlight distinct immune and haematological profiles in UTI patients, depending on the infecting organism. Conclusion: The UTI patients exhibited lower Hb and higher WBC, platelet count, MPV, RDW, PDW and PCT compared to controls. Gram-positive UTI infections showed higher WBC count, platelet counts, RDW, PDW and PCT, but lower MPV compared to gram-negative UTI infections. E. coli and Enterococcus were the predominant gram-negative and gram-positive pathogens, respectively, reflecting distinct haematological profiles. These results highlight distinct immune and haematological profiles in UTI patients, depending on the infecting organism.

Outcome prediction for surgical patients with sepsis may be conducive to early aggressive interventions. In several studies, changes in the level of numerous biomarkers like red cell distribution width (RDW), platelet count (PC), mean platelet volume (MPV), and platelet distribution width (PDW)have been demonstrated to be associated with mortality in critically ill patients. We aimed at investigating the prognostic significance of dynamic changes in RDW, PC, MPV, and PDW in surgical patients with sepsis. We prospectively enrolled 110 surgical patients of sepsis in our study admitted to the surgical ward and ICU. We measured RDW, PC, MPV, and PDW on days 1, day 4, and day 8. Receiver operating characteristics (ROC) were generated for prognostic validation of these parameters and mortality in surgical patients with sepsis. Results: We found that higher RDW and PDW on day 1 amongnon-survivors as compared to survivors on day 1 were significantly associated with mortality. ROC curves showed that RDW and PDW on day 1 could be used to predict mortality in surgical patients with sepsis and it was dynamic changes in PC on day 4 and day 8 along with a change in MPV on day 8, which was significantly associated with mortality. The major findings of our study were baseline value of RDW and PDW on day 1 and continuous decrease in PC and increase in MPV over one week weresignificantly associated with mortality. So, it is better to monitor dynamic changes in PC and MPV in combination with baseline RDW and PDW. So, these parameters can bepromising markers to assess prognosis in surgical patients with sepsis.

Objectives We conducted this study to evaluate the diagnostic value of hematologic markers for moderate to severe obstructive sleep apnea syndrome (OSAS). Methods We performed the study using medical records from our sleep disorders center. We collected information regarding obstructive apnea-hypopnea index (oAHI), age, sex, body mass index, and complete blood counts with differential counts [white blood cell (WBC) count, neutrophil count, lymphocyte count, red blood cell distribution width (RDW), mean platelet volume, platelet count, platelet distribution width (PDW), neutrophil-lymphocyte ratio, and platelet-lymphocyte ratio]. We excluded patients who were younger than 2 years, older than 14 years, obese/underweight, and those who had a hematologic or severe medical illness. Results We assessed records from 57 patients (7.98±3.25 years old, 35 men). We classified the subjects into three groups based on their oAHI scores, as follows: normal (oAHI<1), mild OSAS (1≤oAHI<5), and moderate/severe OSAS (oAHI≥5). Using a multivariate multinomial logistic regression model (pseudo R2=0.33), we found significant differences among the groups in RDW [moderate/severe OSAS vs. mild OSAS, adjusted odds ratio (OR): 8.77, p-value: 0.03], PDW (mild OSAS vs. normal, adjusted OR: 1.05, p-value: 0.04), and WBC (moderate/severe OSAS vs. normal, adjusted OR: 1.42, p-value: 0.03). Conclusions RDW, PDW, and WBC had diagnostic value for moderate/severe OSAS in our study. Further prospective and validation studies are required to develop a screening tool for moderate/severe OSAS in children and adolescents. Keywords: Children, Sleep apnea syndrome, Red cell distribution width, Platelet distribution width

Platelet activation and inflammation are the related mechanisms of pathogenesis in obstructive sleep apnea syndrome (OSAS). Mean platelet volume (MPV) and platelet distribution width (PDW) are the markers of platelet activation. C-reactive protein (CRP) and red cell distribution width (RDW) in relation to the inflammation in OSAS might be increased. We aimed to investigate the correlation of the MPV, PDW, and RDW levels with the severity of OSAS. We included 98 patients with suspected OSAS, evaluated by polysomnography. According to their apnea-hypopnea index (AHI), patients were divided into group A (n = 20; 20.4%) with AHI below 5/h; group B (n = 15; 15.3%) with AHI 5-14.9/h; group C (n = 26; 26.5%) with AHI 15-29.9/h; and group D (n = 37; 37.8%) with AHI ≥30/h. Hemoglobin, MPV, PDW, and RDW were measured using an automated blood cell counter. BMI and age showed a statistically significant and gradual increase in AHI groups. Hemoglobin, platelet, CRP, MPV, and RDW values did not differ between AHI groups. PDW was significantly higher in group D (mean value 14.4 ± 1.8) than in group A (13.2 ± 0.5) (P < 0.001). Epworth sleepiness scale was significantly higher in group D (9.9 ± 5.5) than in group A (5.6 ± 3.5), B (9.2 ± 3.6) and C (6.6 ± 3.8) (P = 0.005). When the four groups were compared, group D had the lowest minimum SpO2 value [group A (89.4 ± 3.0), B (86.7 ± 4.2), C (81.2 ± 6.4), and D (68.2 ± 13.0)]. There was a statistically significant correlation between AHI and age (r = 0.35, P < 0.001), BMI (r = 0.31, P = 0.003), PDW (r = 0.28, P = 0.006), and Epworth sleepiness scale (r = 0.29, P = 0.007). However, AHI was not correlated with CRP, MPV, and RDW. PDW is higher in severe OSAS and is correlated with different parameters of breathing function during sleep. The severity of OSAS was not correlated with CRP, MPV, and RDW. These findings show an evidence for platelet activation in OSAS and suggest that PDW might be related markers of OSAS severity.

The main aim of this study is to investigate complete blood count parameters in order to distinguish between benign and malignant causes of bleeding in patients who presented to the emergency department with gastrointestinal bleeding. In our study, we assessed 455 patients who were admitted to the emergency department between January 2022 and December 2023 with upper and lower gastrointestinal bleeding. Patient's data were analyzed retrospectively. The diagnosis of malignancy was established following pathological investigation. The study investigated the relationship between benign and malignant causes and different components of the complete blood count, including white blood cells, neutrophils, lymphocytes, monocytes, red cell distribution width (RDW), platelet distribution width (PDW), mean platelet volume, platelets, neutrophil-lymphocyte ratio, lymphocyte-monocyte ratio, thrombocyte-lymphocyte ratio, RDW-platelet ratio, and mean platelet volume-platelet ratio. t test and Mann Whitney U test used for parametric and nonparametric scale values. cutoff points, sensitivity and specificity, and area under curve detected with receiver operating characteristic (ROC) curve. Of the 455 patients who applied to the emergency department, 382 (84%) had gastrointestinal bleeding due to benign causes, and 73 (16%) had malignant causes. RDW (P = .008) value was found to be significantly higher in bleeding due to malignant causes. The PDW (P = .017) value was significantly higher in bleeding associated with benign causes. No relationship was found between the other parameters. The ROC curve study revealed that the RDW had the highest diagnostic accuracy for predicting malignancy at a threshold of 14.1, and the area under the curve was 0.654 (95% CI: 0.578-0.729). The ROC curve analysis demonstrated that PDW exhibited the maximum diagnostic accuracy for predicting benign causes at a threshold of 13.8, with an area under the curve of 0.590 (95% CI: 0.502-0.678), showing moderate accuracy. The sensitivity and specificity of the RDW was 86% and 39%, respectively. The sensitivity and specificity of the PDW was 63.95% and 58%, respectively. Our study has shown that the values of RDW and PDW can be valuable in predicting malignant and benign etiology in patients admitted to the emergency department with gastrointestinal bleeding.

The quantitation of platelets in peripheral blood is a well-recognized tool. Recently, new indices related to erythrocytes and platelet counts have been provided by hematologic analyzers. Concerning the platelet parameter, the three important parameters are plateletcrit (PCT), mean platelet volume (MPV), platelet distribution width (PDW). The purpose of the study is to investigate these three parameters to define their expected values and the correlation with the parallel red blood cell parameters, hematocrit (HCT), mean corpuscular volumer (MCV), and red blood cell distribution width (RDW). Blood samples form 215 volunteers were analyzed for platelet parameters using Technicon H*3. The average (mean +/- SD) values obtained with expected ranges were PCT 0.24 +/- 0.05% (range, 0.23-0.24%), MPV 12.79 +/- 5.91 fL (range, 12.39-13.19 fL), and PDW 46.79 +/- 2.70% (range, 46.61-46.97%). Concerning the correlation between PCT, MPV, and RDW and their parallel red blood cell parameters, there was no significant correlation between PCT and HCT, and between MPV and MCV. However, there was a significant correlation between PDW and RDW. It seems that the anisocytosis of red blood cells and platelets might co-occur. However, these data are basic observations; further in-depth evaluation of the platelet parameters is recommended.

BackgroundObstructive sleep apnea syndrome (OSAS) is characterized by repetitive collapse of the upper airway during sleep. Red blood cell distribution width (RDW) increases platelet activation and has been reported as an independent predictor of adverse outcomes in the general population and is believed to be associated with cardiovascular morbidity and mortality. We evaluated RDW, mean platelet volume (MPV), and platelet distribution width (PDW) as a severity index in OSAS and the relationship between carotid intima media thickness and pulmonary hypertension.Material/MethodsThe study population consisted of 99 patients who were admitted to the sleep laboratory. Based on the apnea-hypopnea index, patients were grouped into 3 OSAS severity categories. Morning blood samples were withdrawn from patients after a 12-hour fasting period. MPV, PDW, and RDW were measured in a blood sample. Bilateral common carotid arteries of the patients were scanned.ResultsNinety-nine patients – 73 with OSAS and 26 simple snoring control cases – were included. Mean values of MPV, PDW, and RDW were similar in patients compared to simple snoring subjects in the control group (p=0.162, p=0.656, p=0.091). RDW showed an inverse correlation with mean desaturation and lowest desaturation (p<0.01). Body mass index, apnea-hypopnea index, pulmonary artery pressure, and desaturation time under 90% were positively correlated with RDW (p<0.05). MPV, PDW, and carotid intima media thickness had no correlation with any other parameters.ConclusionsThe study showed a positive relationship between RDW and the apnea-hypopnea index and systolic pulmonary hypertension in patients with OSAS.

Objective To analyze the correlations between serum ferritin (SF) and some blood routine indexes in plateletpheresis donors, so as to screen blood routine indexes which can indicate SF level.Methods 111 plateletpheresis donors from October 2010 to February 2011 in Foshan central blood station were selected. These donors' SF and blood routine indexes were measured by enzyme-linked immunosorbent assay (ELISA) and blood cell analyzer respectively. SPSS statistics 17. 0 was used to analyze the correlations between SF and red blood cell (RBC), white blood cell (WBC), platelet (PLT), hemoglobin (Hb), mean corpuscular volume (MCV), red blood cell distribution width (RDW), mean platelet volume (MPV), platelet-large cell ratio (P-LCR) and platelet distribution width (PDW). Results SF level of plateletpheresis donors was (66. 81 ±36. 63) μg/L and all the donors' RBC, Hb, WBC and PLT were within the normal range. RDW and PLT were negatively correlated to SF (r= - 0. 347, and -0. 315 respectively; P<0. 05), RDW was positively correlated to PLT (r=0. 265, P<0. 05). Conclusion RDW and PLT can indicate SF level in plateletpheresis donors. Paying extra attention to RDW and PLT can contribute to search for latent iron deficiency donors whose RBC and Hb are within the normal range. Key words: apheresis platelets serum ferritin; correlation

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia due to insulin deficiency or resistance, often accompanied by systemic inflammation and vascular complications. Hematological parameters and inflammatory markers derived from complete blood counts (CBC) may reflect glycemic control and disease progression. This study investigates the association between HbA1c levels and hematological indices, including NLR (neutrophil-to-lymphocyte ratio), MLR (monocyte-to-lymphocyte ratio), PLR (platelet-to-lymphocyte ratio), RDW (red cell distribution width), MPV (mean platelet volume) and PDW (platelet distribution width), to assess their diagnostic utility in DM. This study aims to identify the association between glycated hemoglobin (HbA1c) levels and a range of hematological parameters, while also evaluating their diagnostic significance in patients with DM. Extensive research has been carried out on hematological parameters and potential systemic inflammatory markers, including NLR and MLR, concerning tumors, cardiovascular diseases and various systemic inflammatory conditions. Nonetheless, only few studies have explored the correlation between DM and these hematological inflammatory markers. The present cross-sectional study was carried out over a period of two months, from December 2024 to January 2025, in a tertiary care center located in Rajkot, India. It included 753 cases, with 424 patients exhibiting HbA1c values within the diabetic range, 182 in the prediabetic range and 147 subjects in the non-diabetic range. Comprehensive data was collected and glycated hemoglobin levels, complete blood count results and inflammatory hematological parameters were analyzed. Statistical analyzes was conducted using the Statistical Package for the Social Sciences version 28.0 software. Univariate analysis using one-way ANOVA and Pearson correlation, followed by multinomial logistic regression were utilized to investigate the relationship between HbA1c and hematological parameters. The red blood cell indices and platelet distribution width were notably lower in diabetic patients, while the monocyte lymphocyte ratio was found to be significantly higher in non-diabetic ones. Correlation analysis between HbA1c levels and hematological parameters indicated several significant yet weak associations. Furthermore, multinomial logistic regression analysis suggested that hematological parameters played a role in differentiating diabetic individuals from prediabetic and non-diabetic ones. Understanding the associations between hematological parameters and HbA1c is important, as these correlations may reveal how glycemic control affects blood cell dynamics and systemic inflammation, thus contributing to effective diagnosis and management.