Biological role of β-arrestin1 in human gastric cancer BGC-823 cells

Abstract

Objective To investigate the effects of β-arrestinl on proliferation, migration, invasion and apoptosis of human gastric cancer BGC-823 cell line. Methods The expression of β- arrestinl in human gastric epithelial cell line GES, human gastric cancer cell line BGC-823, MKN-28 and SGC-7901 was detected by realtime-polymerase chain reaction （PCR） and Western blot. The stable β-arrestinl and negative control interfered BGC-823 cell line were established by RNA interference technology. The cell proliferation, migration, invasion and cell apoptosis of β-arrestinl stable interfered BGC-823 cell line was examined by cell counting, scratch test, Transwell chamber test and flow cytometry assays. The data were analyzed by t test. Results The expression of β- arrestinl in cell line GES, MKN-28, SGC-7901 and BGC-823 was 0. 001 ± 0. 001, 0. 002±0. 000, 0. 003± 0. 002 and 0. 005 ± 0. 000 respectively. The inhibition ratio of proliferation in β-arrestinl interfered BGC-823 cells and negative control cells were -30.2% and 100.0%. The invasion ability was also inhibited, the number of migratory cells was 126. 25±3.24 and 213. 50±6.27 （t=0. 000, P〈0.01）, and the apoptosis rate was （41. 350±1. 053）% and （11. 497±0. 589）% （t=0. 015, P〈0.05）. Conclusions β-arrestinl is highly expressed in gastric carcinoma, and the expression increased along with the malignancy degree. The cell proliferation, migration and invasion is inhibited by interference of β-arrestinl in BGC-823 cells, while the cell apoptosis is promoted. Key words: β-arrestinl; Stomach neoplasms; RNA interference; Tumor cells, cultured