Biological properties of a crude venom extract from the greater weever fish Trachinus draco

Abstract

Crude venom of the greater weever fish, Trachinus draco was analyzed to assess its toxicity, stability and biological properties. The best yield of venom was obtained by extraction in physiological saline of the whole venom apparatus of the fish which were shock-frozen and stored at −70°C. This extract had a mouse i.v. minimum lethal dose of 1.8 μg venom protein per gram mouse and a total of 61,000 minimum lethal doses were obtained from the venom apparatus of one fish. The lethal activity was unstable at room temperature especially at lower protein concentrations. Stability was achieved either by storing the extract at −70°C or by precipitation with ammonium sulfate at 50% saturation. Toxicity of the crude venom was abolished by trypsin treatment. The crude venom did not possess any proteolytic or histamine-releasing activities. The venom caused an outflow of tetraphenylphosphonium from preloaded rat brain particles in a concentration-dependent manner. Like toxicity, this effect was also abolished by trypsin treatment or by keeping the venom at higher temperatures. The crude venom also possessed hemolytic activity with an ec 50 for rabbit erythrocytes of 75 ng/ml venom protein. The hemolytic activity was also sensitive to heat and proteolytic treatment. Rabbit erythrocytes were most sensitive to venom followed by rat erythrocytes. Mouse and cattle erythrocytes were only slightly sensitive, whereas human, chicken and guinea pig erythrocytes were totally resistant.