Abstract
Experiments were carried out to test the accuracy of using amino acid analyses and percentage absorption data of wheat by-products to calculate the amount of amino acid supplementation required to meet the total requirements of the rat, when bran, shorts or middlings were used as the only source of protein in the diet. A soybean meal diet supplemented with methionine was used as a control. Each diet was fed ad libitum to four rats, housed in individual cages, for a 7-day period. Weight gain, feed efficiency and net protein utilization (NPU) were used as criteria of the adequacy of the diets. Rats fed the supplemented shorts and middlings diets showed as good growth, feed efficiency and NPU values as those on the soybean meal diet. Weight gain and NPU were somewhat lower on the supplemented bran diet. These results may be partly explained on the basis of the lower content of total digestible nitrogen and the higher fiber content of the bran diet. Omission of any of the essential amino acids from the amino acid supplement calculated to be required in the bran diet caused a marked decrease in growth and feed efficiency.
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