Abstract
The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to show the potential application of biological autoluminescence (BAL) for monitoring of pulsed electric field (PEF) biological effects on yeasts. The yeast cell culture (Saccharomyces cerevisiae) is exposed to the pulsed electric field (pulse duration: 0.1-5 ms, pulse number: 1-10, electric field strength: $3 kV.cm^{-1}$) and the BAL is monitored during exposure. The results indicate detectable differences in BAL dynamics when PEF is applied. Due to its noninvasive and label-free application, the BAL could be used as a beneficial tool for monitoring of PEF biological effects.
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