Abstract
Incubation of 4(or 5)-diazoimidazole-5(or 4)-carboxamide (Diazo-ICA) at 37° with isolated rabbit platelets induces release of 5-hydroxytryptamine (5-HT). This release occurs only in the presence of a trace of calcium ions and the effect is independent of the presence of other plasma components. The release is temperature-dependent, being appreciably lower at room temperature than at 37° and almost negligible at 4°. These results imply that the mechanism of release with Diazo-ICA differs from that with reserpine or bacterial pyrogenic lipopolysaccharide. 4(or 5)-Dialkyltriazenoimidazole-5 or 4)-carboxamides, prepared by coupling Diazo-ICA with corresponding dialkylamines, cause only slight release of 5-HT from platelets, and the release is independent of the presence of calcium ion. In contrast, 4(or 5)-aminoimidazole-5(or 4)-carboxamide (the parent compound of these derivatives), 2-azahypoxanthine (a cyclized product of Diazo-ICA) and S-(5(or 4)-carbamoyl-4(or 5)-imidazolyl azo)cysteine (a coupling product of Diazo-ICA and cysteine) release no 5-HT from platelets. The other diazonium compound tested, diazobenzene sulfonate and diazobenzenesulfonamide, also cause no release.
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