Biological activities of new secondary metabolite produced by &lt;em&gt;Streptomyces badius&lt;/em&gt;

Jolanta Solecka,Robert Kawęcki,Joanna Ziemska,Aleksandra Rajnisz-Mateusiak,Katarzyna Jakubiec-Krześniak,Magdalena Postek,Dorota Gudanis

doi:10.3390/ecsoc-20-b006

Abstract

Secondary metabolites obtained from Actinomycetales provide a potential source of many new antibacterial, antitumour, antifungal, antiviral, antiparasitic compounds and others. The majority of discovered substances are widely used as medicines for combating multidrug-resistant Gram-positive and Gram-negative bacterial strains, as well as in veterinary practice, agriculture and industry. Members of the Streptomyces genus are outstanding producers of already known secondary metabolites. In our study we isolated a new metabolite from Streptomyces badius fermentation broth. This compound, 2-acetamido-5-hydroxybenzoic acid (1) was already described in literature, however, not yet discovered as Streptomyces badius product. The compound was firstly isolated from fermentation broth and purified by chromatography methods (ion exchange resin DowexWX40, Solid Phase Extraction C18 Polar Plus; HPLC, dC18). Then, the chemical structure of metabolite was determined by 1H NMR, 13C NMR and 2D homo- and heteronuclear (1H-13C HSQC, 1H-13C HMBC) NMR. The molecular formula of (1), C9H9NO4 was identified by high resolution ESI-MS. The chemical structure was analyzed by the UV and IR methods, as well. Biological activities of 2-acetamido-5-hydroxybenzoic acid were evaluated. The compound shows moderate DD-peptidase 64-575 inhibitory activity as well as antioxidative properties (ABTS radical scavenging). Such chemical moiety may serve as model compound for further modern drug discovery and be a source of active substance in anti-ageing cosmetics.

Highlights

Search for bioactive compounds from nature play crucial role in fashioning new therapeutic agents
Strain: Streptomyces badius ATCC 19888 was isolated from the soil in Kaukasus (Russia)
The DPPH and ABTS radicals scavenging activity was assayed based on methods previously described [6]

Summary

Introduction

Search for bioactive compounds from nature play crucial role in fashioning new therapeutic agents. Secondary metabolites have major importance in drug discovery process. They are diverse and unusual in their chemical structures and may be used as scaffolds for further modifications. Actinomycetes are main producers of bioactive metabolites [1,2]. Actinomycetes are the most widely distributed groups of microorganisms in nature. They can be found in various environments such as soil and water. Their metabolites are active against bacteria, viruses, fungi, parasites and cancer cells [3].

Materials and Methods

Results