Abstract

A novel synthetic polymer (ethylene-vinyl acetate copolymer; EVA) was used to immobilize acclimated sewage sludge for H 2 production under anaerobic conditions. Using sucrose as the sole carbon substrate, the resulting EVA-immobilized cells achieved an optimal H 2 production rate ( v H 2 ) of 488 ml H 2/g VSS and the best substrate-based yield ( Y H 2 / sucrose ) of 1.74 mol H 2/mol sucrose. Operation at a temperature of 40 °C resulted in the most efficient H 2 production. Acclimation of the sewage sludge allowed up to 3-fold enhancement on the performance of H 2 production. Kinetic studies show that a Monod-type model is able to describe the dependence of specific H 2 production rate on sucrose concentration. The immobilized cells maintained stable and efficient H 2 production during 15 repeated runs, indicating excellent durability and stability of the immobilized-cell system. The composition of soluble metabolites was found to be a reliable indicator for the efficiency of biohydrogenation.

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