Abstract
The process of producing oil from fresh fruit bunches produces palm oil effluent (POME). Good processing of POME is needed to prevent emissions that can damage the environment. POME can be used as a substrate to produce biohydrogen gas. This study aims to treat palm oil liquid waste-producing biohydrogen by dark fermentation using Clostridium butyricum. The initial stage is the addition of NaOH to POME which functions to degrade lignin and stop methanogenic bacteria. Then the hydrolysis method was carried out during dark fermentation using Clostridium butyricum with varying concentrations of NaOH (0.3; 0.5; 1% w/v POME) and hydraulic retention time. The results showed the highest biohydrogen production was obtained at a concentration of NaOH 0.3% w/v with a hydraulic retention time of 32 hours with an amount of 2824.2 mg H2/L POME.
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