Bioethanol production from Codium tomentosum residue

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ABSTRACT The present study investigated the feasibility of using residual biomass of marine macroalgae (Codium tomentosum) for bioethanol production. Influence of various parameters was analyzed for optimization of bioethanol production. Pre-treatment was performed with different acids, H2SO4 concentrations, temperatures, and reaction times. Maximum reducing sugar yield achieved was 16.45 ± 0.33 g/l with 4% of H2SO4 at 100°C for 60 min. Cellulase produced from Trichoderma reesei is used for the hydrolysis of biomass. Cellulase activity was estimated at various pH, temperatures, and reaction times. The partially purified cellulase was found to have more activity at 30°C for 48 h of cultivation at pH 5. Enzymatic hydrolysis was analyzed with various biomass concentrations, enzyme concentrations, temperatures, and reaction times. Fermentable sugar yield of 17.05 ± 0.25 g/l was obtained using 5% (w/v) biomass with 4% (w/v) enzyme at 50°C for 48 h. The fermentable sugar was converted to bioethanol by fermentation using Saccharomyces cerevisiae with different parameters such as different pH, temperatures, and reaction times were executed. The maximum bioethanol yield of 4 ± 0.33 g/l was obtained at 30°C for 72 h at pH 5. Further, the bioethanol content was characterized using GC-MS. Thus, the results shows that Codium tomentosum is an excellent biomass resource that can be used as a renewable feedstock for the production of bioethanol.