Abstract
The aim of this study was to determine the biodiversity of PHB producing bacteria isolated from soils where fruit and vegetable are cultivated (onion, grape, olive, mulberry and plum) in Aydın providence. Morphological, cultural, biochemical, and molecular methods were used for bacteria identification. These isolated bacteria were identified by 16S rRNA sequencing and using BLAST. The following bacteria Bacillus thuringiensis (6), B acillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. The Maximum Likelihood method was used to produce a molecular phylogenetic analysis and a phylogenetic tree was constructed. These bacteria can produce polyhydroxybutyrate (PHB) which is an organic polymer with commercial potential as a biodegradable thermoplastic. PHB can be used instead of petrol derivated non-degradable plastics. For this reason, PHB producing microorganisms are substantial in industry.
Highlights
Plastics products are indispensable from automobiles to medicine in our lives
Isolation and identification of PHB producing bacteria from environments such as soil, sea water, aerobic and anaerobic sewage is important since these bacteria
The garden soil samples used in this study were collected from 0-15 cm layer
Summary
Plastics products are indispensable from automobiles to medicine in our lives. We use plastics and synthetic polymers produced from petrochemicals. With the advancement of biotechnological research, the production of environmentally friendly plastic has been on the rise It has good oxygen permeability and ultraviolet resistance. Isolation and identification of PHB producing bacteria from environments such as soil, sea water, aerobic and anaerobic sewage is important since these bacteria López-Cortés et al [8] isolated PHB-producing bacteria in a polluted marine microbial mat and identified using 16S rDNA gene sequence. As result, they found Bacillus, Staphylococcus, Paracoccus, Micrococcus, Rhodococcus and Methylobacterium. The garden soil samples used in this study were collected from 0-15 cm layer
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