Abstract
Novel bacterial strain MF-1, able to grow using melamine formaldehyde (MF) resin as main carbon and nitrogen source, is described and characterized. Strain MF-1 was isolated from wastewater effluent of an aminoplastic industrial plant. Growth rate kinetics of the strain on MF showed a maximum specific growth rate ( μ max ) of 0.83 cells ml −1 h −1 and K s of 7.18 cells ml −1 . Mineralization of MF by strain MF-1 was confirmed by the decrease in dissolved oxygen (DO), release of ammonia, and detection of intermediate metabolites during biodegradation. Melamine, cyanuric acid , and biuret were detected as intermediate metabolites in the culture filtrate, suggesting that biodegradation of MF by strain MF-1 proceeds via successive deamination reactions of melamine to cyanuric acid, which is hydrolyzed to biuret and finally to NH 3 and CO 2 . Based on 16S-rDNA sequence analysis, strain MF-1 had a similarity of 97% to Micrococcus sp. MN 8.1d. However, the high bootstrap value obtained in the phylogenetic analyses suggests that this is a novel strain.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Biodeterioration & Biodegradation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
