Abstract
The oil spills in the vicinity of the Garo sulfidic springs in Golgir region, MIS oilfield, Iran, were investigated for halotolerant/halophilic microorganisms capable of crude oil biodegradation. Screenings resulted in an isolate capable of growth on crude oil of the local Asmari reservoir in media containing 30–100 g/L NaCl at 28 °C. The isolate, designed as KWPA-12, was an aerobic, motile, oxidase and catalase positive, Gram-negative rod. It was shown that strain KWPA-12 can utilize crude oil as the carbon and sulfur sources with a significant efficiency. The Asmari crude oil biodegradation in 90 g/L NaCl containing media was investigated by turbidity measurement, GC-FID and TPH analyses. The results showed that the aliphatic hydrocarbons including: small C11–C12 and long chains: C31–C33, are mostly favored by strain KWPA-12. Anthracene: a tri-ring and one of the most dominant poly-aromatic compounds of Asmari crude oil, was reduced down to about 1.1% of the starting moiety. Phylogenetic investigations performed on the partial 16S rRNA gene sequence indicated that the strain belongs to the genus Halomonas and it fell into the Halomonas elongata clade.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
