Abstract
This paper presents a first scale up under non-sterile conditions of the biodegradation process of 2-naphthalensulfonic acid polymers (NSAP) contained in a petrochemical wastewater by two white-rot fungi (Bjerkandera adusta and Pleurotus ostreatus). The biodegradation experiment was conducted first in flasks and then in packed-bed bioreactors filled with inert and biodegradable carriers (straw), the latter acting as both physical support and carbon source. Reactor inoculated with P. ostreatus attached on straw worked under non-sterile conditions for three months showing 30 ± 5% NSAP degradation. Respirometric tests showed that the fungal treatment was also able to significantly increase the biodegradable fraction of the wastewater COD, which rose from 9% to 40%. It was observed that the fungal degradation of the straw in the bed releases non-biodegradable by-products. Taking into account this contribution to nbCOD, the combined treatment of fungi and activated sludge could theoretically be able to reduce the original COD by up to 73%.
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