Abstract
Dengue fever is a prevalent and rapidly spreading disease. It is best controlled by controlling its vectors. Aedes aegypti and Aedes albopictus are the primary and secondary worldwide vectors respectively, which are bred in peridomestic man-made water containers. Biological control is the most effective and sustainable method as there is no resurgence effect and does not harm humans. Our study includes the use of extracellular proteins of Xenorhabdus nematophilus, a gram negative bacterium widely used as biocontrol agents belonging to the family enterobacteriaceae. The mortality rates of IVth instar larvae Aedes aegypti when treated with 250 µg of extracellular proteins of Xenorhabdus nematophilus recorded to be 40% after 72h of exposure. The rate of mortality was observed minimum even at higher concentration. Optimization of the medium through Response Surface Methodology showed that there was an increase in the production of extra cellular proteins. These proteins played a very important role in the control of Aedes aegypti. The rate of mortality was recorded to be 92% when treated with 200 µg of extracellular proteins within 48h of treatment. Our research proved that the media optimization enhanced the production of extracellular proteins in the Xenorhabdus nematophilus and it can be used as a bio control agent for the control of Dengue vector.
Highlights
Dengue fever is caused by any of the five serotypes’(DENV 1, 2, 3, 4, and 5) of dengue virus belonging to the family Flaviviridae and transmitted mainly by the insect vector Aedes aegypti, of Culicidae family
Our study includes the use of extracellular proteins of Xenorhabdus nematophilus, a Gram-negative bacterium widely used as biocontrol agents belonging to the family Enterobacteriaceae
Our research proved that the media optimization enhanced the production of extracellular proteins in the X. nematophilus and it can be used as a biocontrol agent for the control of dengue vector
Summary
(DENV 1, 2, 3, 4, and 5) of dengue virus belonging to the family Flaviviridae and transmitted mainly by the insect vector Aedes aegypti, of Culicidae family. The. Vani et al / Journal of Applied Pharmaceutical Science 8 (11); 2018: 131-139 foraging nematodes locate the prey insect pests and penetrate to the hemolymph, acting as vectors for the injection of their symbiotic bacteria. Vani et al / Journal of Applied Pharmaceutical Science 8 (11); 2018: 131-139 foraging nematodes locate the prey insect pests and penetrate to the hemolymph, acting as vectors for the injection of their symbiotic bacteria These bacteria do not kill the insects if ingested but do so quickly if they are introduced to the hemolymph, either via nematode infection or via syringe injection of laboratory cultures. This study includes the biological control of A. aegypti larvae using extracellular proteins from X. nematophilus and the enhancement of proteins by response surface methodology (RSM)
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