Abstract
The pathogenic strains of Fusarium spp. wilt entire hectares of agriculturally important crops, and biological control is the most advantageous method to halt this problem. In this study, fourteen soil surface fungi were isolated using serial dilution and initial inoculation in synthetic nutrient-poor agar (SNA), Czapek Dox agar (CDA), and malt extract agar (MEA). The soilborne fungal isolates were morphologically identified into four genera: Aspergillus, Curvularia, Dictyuchus, Trichoderma, while two were identified as mycelia sterilia. We performed dual culture and best inoculation method to test the biocontrol activity of the 14 soil surface fungi against Fusarium verticillioides, Fusarium moniliforme, and Fusarium sp. Both methods revealed that soil surface fungi could inhibit the growth of phytopathogenic fungi. Aspergillus sp. 3 inhibited the growth of three pathogenic fungi when planted first in best inoculation, while Trichoderma sp. inhibited the growth of Fusarium sp. in both methods, and F. verticillioides when planted first in the best inoculation method.
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