Abstract
Silicon has been widely used as a material for microelectronic for more than 60 years, attracting considerable scientific interest as a promising tool for the manufacture of implantable medical devices in the context of neurodegenerative diseases. However, the use of such material involves responsibilities due to its toxicity, and researchers are pushing towards the generation of new classes of composite semiconductors, including the Silicon Carbide (3C-SiC). In the present work, we tested the biocompatibility of Silicon and 3C-SiC using an in vitro model of human neuronal stem cells derived from dental pulp (DP-NSCs) and mouse Olfactory Ensheathing Cells (OECs), a particular glial cell type showing stem cell characteristics. Specifically, we investigated the effects of 3C-SiC on neural cell morphology, viability and mitochondrial membrane potential. Data showed that both DP-NSCs and OECs, cultured on 3C-SiC, did not undergo consistent oxidative stress events and did not exhibit morphological modifications or adverse reactions in mitochondrial membrane potential. Our findings highlight the possibility to use Neural Stem Cells plated on 3C-SiC substrate as clinical tool for lesioned neural areas, paving the way for future perspectives in novel cell therapies for neuro-degenerated patients.
Highlights
Among the different types of currently available stem cells, neural stem cells (NSCs) have been widely applied as suitable cells for neuro-regeneration
Olfactory Ensheathing Cells (OECs) and DP-NSCs have been recognized as an interesting alternative source of stem cells for cellular transplantations strategies[8,9] and the management of neurodegenerative diseases and Spinal Cord Injury, one of the most devastating forms of injury leading to disability and death[9]
The aim of the present work was to test the biocompatibility of Si and 3C-SiC in an in vitro model of human neurons derived from dental pulp mesenchymal stem cells, and in OECs derived from mouse olfactory bulbs
Summary
Among the different types of currently available stem cells, neural stem cells (NSCs) have been widely applied as suitable cells for neuro-regeneration. Previous research showed the innate neurogenic potential of DPSCs, which are derived from the neural crest[7] Another type of interesting cells for their ability to promote axonal regeneration and functional restoration in the lesioned neural areas are the Olfactory Ensheathing Cells (OECs). The combination of stem cells and nanotechnologies seem to be a promising approach for the development of clinical translatable cell-based therapies enhancing neural repair Following this standpoint, nanotechnology and regenerative medicine strategies represent a future perspective for the development of novel therapies that would reach from bench to bedside to serve the neuro-degenerated patients. Nanotechnology and regenerative medicine strategies represent a future perspective for the development of novel therapies that would reach from bench to bedside to serve the neuro-degenerated patients In this context, many candidate semiconducting materials for biotechnological applications have been investigated for biocompatibility and sensing potentiality. Silicon Carbide (3C-SiC) has been proven to be a good substrate for this purpose, being bio- and hemo-compatible, and usable for the manufacture of implantable devices[10,11]
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