Abstract
Gradiflow™ is a membrane-based preparative electrophoresis technology in which separations are achieved using the dual strategy of molecular charge and size. The distinguishing features of this technology are a set of polyatrylamide membranes and the application of ail electrical potential across these membranes. The use of these features allows the selective removal of contaminants from biological fluids. Gradiflow™ has previously been shown to have potential in renal dialysis and has demonstrated the effective removal of nitrogenous waste metabolites and phosphate ions from human plasma and whole blood samples. This raised the question of the biocompatibility of the membranes and the effect of voltage on cellular and biochemical components of human plasma and blood. To address this issue, tests on haemolysis, coagulation, complement and cellular activation were conducted. Our results showed that the Gradiflow™ did not have significant effects on haemolysis, coagulation and complement activation. Flow cytometry results indicated that platelets and white cells were not adversely affected. These results indicate that Gradiflow™ has significant potential in the treatment of renally compromised patients and may have therapeutic uses in the treatment of disease states which require the removal of toxic material from body fluids.
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