Biochemical evaluation of depigmentation in C57/BL6 mice and its treatment by Psoralea corylifolia. Linn (Bakuchi) seed oil and seed extracts

Abstract

Skin is the largest organ of the body it protects us from several kinds of environmental hazards and also works as a connective unit between environment and individual. Skin can react in several ways against emotional factors. Alopecia areata and vitiligo are such diseases which effect individuals and their social environments. Vitiligo is a depigmented disorder where complete loss of melanocytes takes place. There are six major factors which define aetiology of vitiligo. Present study is an endeavour to establish a treatment which can change the painful allopathic treatment process of UV exposure, which results in post treatment hazards such as psores, inflammation, and pain. In the present study C57/BL6 mice were selected. The groups are divided in protocol 1; control, toxicant a and toxicant b, protocol 2; 100 mg/kg b.w, 200 mg/kg b.w and 300 mg/kg b.w of ethanolic extract of Psoraleya corylifolia. Linn seeds, and 100 mg/kg b.w, 200 mg/kg b.w and 300 mg/kg b.w of petrolium ether extract of Psoraleya corylifolia. Linn seeds. Topically applied groups of Psoraleya corylifolia Linn. seed oil for 3 weeks, 5 weeks and 7 weeks. The last group was petrolium ether 100 mg/kg b.w+ seed oil topically applied for 5 weeks. Further SOD (superoxide dis mutase), GSH (reduced glutathione), GPx (glutathione per oxidase), MDA (Malone di aldehyde), vitamin E were estimated on the depigmented skin samples. The study concluded that petrolium ether 100 mg/kg b.w showed better results for SOD and MDA, while for GSH and vitamin E ethanolic extract 300 mg/kg b.w showed better results in comparisons to the earlier one. The study successfully justified the hypothesis and the animals were relaxed during the study.