Abstract
BackgroundPodophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from P. hexandrum rhizome.MethodsThe ethyl acetate and ethanol extracts from P. hexandrum rhizome were analyzed by GC-MS (gas chromatography–mass spectrometry), and the antioxidant capacity of the extracts and the components was tested by using the DPPH (2, 2-diphenylpicrylhydrazyl) and FRAP (Ferric reducing/antioxidant power) assays.ResultsThe rhizome extracts had greater antioxidant capacity than the petiole extracts in DPPH and FRAP assays. About 16 kinds of main reactive oxygen components were identified in the extracts. Components of PADE (Phthalic acid, diisobutyl ester), BADE (1,2-Benzenedicarboxylic acid, diisooctyl ester), Polyneuridine, PODD (Podophyllotoxin, deoxy), β-Sitosterol and POD (Podophyllotoxin) showed the antioxidant capacity in some degree. PODD, POD, and Polyneuridine showed stronger antioxidant capacity with the IC50 and FRAP values of 9.61 ± 0.81 and 2923.98 ± 21.89 μM, 9.98 ± 0.24 and 2847.27 ± 14.82 μM, and 13.37 ± 0.35 and 2404.32 ± 36.88 μM, respectively, than the positive control ASA (Ascorbic acid) with the values of 60.78 ± 1.22 and 1267.5 ± 30.24 μM (P < 0.01).ConclusionsPODD, POD, and Polyneuridine are very critical for the antioxidant capacity in the extract of P. hexandrum rhizome. These results provide useful biochemical basis and information for the potential use of this plant.
Highlights
Podophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic
The statistical analyses showed that the rhizome extracts had greater antioxidant capacity than the petiole extracts and the ethanol extracts had greater antioxidant capacity than the ethyl acetate extract both in DPPH and FRAP assays (p < 0.01)
Separation and identification of rhizome extracts In order to further study the biochemical compositions in P. hexandrum rhizome, the extracts of the ethyl acetate and ethanol were separated and identified by GCMS
Summary
Podophyllum hexandrum Royle (P. hexandrum) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from P. hexandrum rhizome. Podophyllum hexandrum Royle (P. hexandrum), commonly named Himalayan Mayapple, is a perennial herb that grows in the Himalayan region and the southwest of China [1,2]. Since 1940 P. hexandrum resin has been used topically for various skin lesions such as warts and condylomas [3]. POD is a natural product mainly existing in P.hexandrum rhizome. It has been used in the treatment of genital infection to sterilize noncervical human papilloma virus [4]. Penile warts can be safely treated with 0.5–2.0% podophyllin self applied [5]. Goel et al [6] reported a significant antitumour effect at
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