Abstract
1. 1. The biochemical composition of the peritrophic membrane of both the tobacco hornworm ( Manduca sexta) and Douglas fir tussock moth ( Orgyia pseudotsugata) larvae has been studied. 2. 2. Although the total protein content of the membranes was the same, the amino acid compositions were distinctive for each larval peritrophic membrane. 3. 3. Major M. sexta peritrophic membrane proteins were detected with apparent molecular weights from 32,000 to 210,000 daltons. O. pseudotsugata peritrophic membrane proteins ranged in molecular weight from 20,000 to 220,000 daltons. 4. 4. Periodic acid Schiff (PAS) staining indicated that both membranes contain glycoproteins. 5. 5. The peritrophic membrane of O. pseudotsugata contains approximately twice the amount of hexosamines as that of M. sexta. Both glucosamine and galactosamine are at higher levels in the O. pseudotsugata membrane than in that of M. sexta.
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