Biochemical and genetic characterization of the β-lactamases of Y. aldovae, Y. bercovieri, Y. frederiksenii and ‘ Y. ruckeri’ strains

Abstract

The β-lactamases of five strains each of Y. aldovae and ‘ Y. ruckeri’, and 10 strains each of Y. bercovieri and Y. frederiksenii were examined phenotypically and genetically. β-Lactamase activity and induction assays and SDS-PAGE were applied for phenotypic characterization of these enzymes. Genotypically, PCR experiments applying degenerated primer pairs for the detection of AmpC β-lactamase genes were performed. All yersiniae yielded specific amplification products for ampC and all these strains expressed β-lactamases. Each species produced its own, species-specific AmpC β-lactamase. Inducibility of these enzymes was shown for Y. bercovieri, but not for the low-level enzyme producing species Y. aldovae and ‘ Y. ruckeri’. In contrast to these species, induction tests for Y. frederiksenii revealed heterogenous results. Whereas the β-lactamases of 6 of 10 strains were inducible, the enzyme activities after induction in the remaining four were similar to those measured without an inducer. In addition to the AmpC enzyme, all Y. frederiksenii strains expressed a second β-lactamase belonging to Ambler class A. The present study enlarges the knowledge about the β-lactamases of four novel Yersinia species that are likely to be involved in human disease. β-Lactamases of Y. aldovae and ‘ Y. ruckeri’ have been characterized for the first time.