Bioavailability of the Digestible Lysine and Valine in Cottonseed and Soybean Meals for Chicks

Abstract

Previous research showed that formulation of chick diets containing cottonseed meal (CSM) using true digestible amino acid (AA) values was superior to formulation based on total AA; however, optimum growth performance could not be obtained from high CSM diets even when formulated on a digestible AA basis. Therefore, two experiments were conducted to determine whether the digestible AA in CSM were totally bioavailable or utilizable for protein synthesis. Soybean meal (SBM) was also evaluated for comparison. True digestibility of Lys and Val in CSM and SBM were determined using the precision-fed cecectomized rooster assay. Bioavailability was then assessed by chick growth assay using purified crystalline AA diets deficient in Lys or Val that were supplemented with varying levels of the test AA, CSM, SBM, or AA mixtures simulating the digestible AA composition of CSM and SBM. Multiple regression slope-ratio methodology indicated that bioavailability of the digestible Lys in CSM and the digestible Lys and Val in SBM did not differ (P > 0.05) from 100% for most response parameters. Bioavailability of the digestible Val in CSM ranged from 78 to 96% depending on response parameter. Bioavailabilities of crystalline Lys and Val in the AA mixtures simulating CSM and SBM were generally lower than the bioavailabilities of the digestible Lys and Val in CSM and SBM. The results of this study indicated that the digestible Lys and Val in SBM and CSM as measured by the precision-fed cecectomized rooster assay are totally or almost totally bioavailable for protein synthesis.