Abstract
Holarrhena antidysenterica seeds were extracted with methanol, dried, and fractioned in to ethyl acetate, n-butanol, and aqueous fractions. A compound isolated from n-butanol fraction using diaion column chromatography followed by high-performance liquid chromatography purification, was characterized as 5-caffeoylquinic acid (chlorogenic acid). The extract and fractions were evaluated for their potential to inhibit α-glucosidase and α-amylase and increase glucose uptake in L6-myotubes. Methanolic extract and n-butanol fraction inhibited the α-glucosidase (IC50 751±15 and 472 ± 8 μg.mL−1) and α-amylase (IC50711 ± 12 and 392 ± 10 μg.mL−1) enzyme. n-Butanol fraction showed maximum increase in glucose uptake at 50 μg.mL−1 by 39.71 ± 3.65%. n-butanol fraction contained 5-caffeoylquinic acid as a major constituent (12.882 ± 1.466 % w/w).
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