Abstract
Spirulina is a microalga known as a superfood which contains high protein and is used in cosmetics, foods, and supplements industry. Collagen hydrolysate is the result of hydrolysis of collagen by protease. This study was aimed to determine the antioxidant activity and inhibition of tyrosynase from Spirulina, collagen hydrolysate and tablet formulated by combination of Spirulina and collagen hydrolysate. This research was conducted in three stages. The first stage was the extraction and hydrolysis of tilapia skin collagen, while the second stage was culturing and harvesting of Spirulina, and the third stage was determination of tablet formulation based on Spirulina and collagen hydrolysate followed by analysis of the antioxidant activity and inhibition of tyrosinase by selected tablet. Three formula with different ratio between Spirulina and collagen hydrolysates namely formula A (25%:53.75%), formula B (28.125%:50.625%), formula C (31.25%:47.5%) and additive such as vitamin C (12.5%), talk (1.25%), magnesium stearate (0.625%), aerosil (1.875%), avicel (5%) were developed. The result showed that formula A was the selected formula meeting the requirements of Ministry of Health based on the physical characteristics. The antioxidant activity of tablet A was 843 ppm and the tyrosinase inhibition was 163.56 ppm.
Highlights
Spirulina is a microalga known as a superfood which contains high protein and is used in cosmetics, foods, and supplements industry
Formulasi tablet yang digunakan meliputi tiga formula dengan perbandingan Spirulina dan hidrolisat kolagen yang berbeda yaitu formula A (25%: 53,75%), formula B (28,125%: 50,625%), formula C (31,25%: 47,5%) dan bahan tambahan seperti vitamin C (12,5%), talk (1,25%), magnesium stearat (0,625%), aerosil (1,875%), serta avicel (5%)
Collagen hydrolysate is the result of hydrolysis of collagen by protease
Summary
Bahan yang digunakan pada penelitian ini adalah Spirulina sp. diperoleh dari Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara Jawa Tengah, kulit ikan nila diperoleh dari danau Toba Sumatra Utara, Walne teknis, NaOH (Merck), akuades, CH3COOH (Merck), 2.2-Diphenyl1-Picrylhydrazyl (DPPH) (Sigma), enzim tirosinase (Sigma), metanol (Merck), sodium dodecyl sulfat-polyacrilamide. Alat yang digunakan meliputi lampu neon Hannoch 40 watt, spektrofotometer (SPECTRO UVVIS), waterbath (Stuart 6L), freeze dryer (Eyela FDU-1200 Japan), Fourier Transform Chromatography Spectroscopy (Bruker Tensor 37 Germany)
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