Abstract
Lipid-based oleaginous microorganisms are potential candidates and resources for the sustainable production of biofuels. This study was designed to evaluate the performance of several alternative bio-based solvents for extracting lipids from yeasts. We used experimental design and simulation with Hansen solubility simulations and the conductor-like screening model for realistic solvation (COSMO-RS) to simulate the solubilization of lipids in each of these solvents. Lipid extracts were analyzed by high performance thin-layer chromatography (HPTLC) to obtain the distribution of lipids classes and gas chromatography coupled with a flame ionization detector (GC/FID) to obtain fatty acid profiles. Our aim was to correlate simulation with experimentation for extraction and solvation of lipids with bio-based solvents in order to make a preliminary evaluation for the replacement of hexane to extract lipids from microorganisms. Differences between theory and practice were noted for several solvents, such as CPME, MeTHF and ethyl acetate, which appeared to be good candidates to replace hexane.
Highlights
Like every other mode of transport, aviation has to respond to the challenges raised by climate change
The oil obtained was analyzed by high thin-layer chromatography (HPTLC)
Lipid classes and by gas chromatography coupled with a flame ionization detector (GC/FID) after to obtain lipid classes and by gasacid chromatography coupled with a flame ionization detector (GC/FID)
Summary
Like every other mode of transport, aviation has to respond to the challenges raised by climate change. The combustion of fuels contributes to the greenhouse effect due to carbon emission. In the face of petroleum resource depletion and concern about the environment, the development of renewable, sustainable and efficient energy sources is being strongly encouraged. Lipid-based biomass is being increasingly considered as an alternative raw material for vehicle fuels. Lipid-based biomasses derived from edible oil crops, such as rapeseed, soybean and maize, form the first generation feedstock, because of their ready availability. The use of first generation biofuels is controversial due to their competition with food. The development of non-food oil plants has been proposed to provide feedstock, such as Camelina and jatropha. A number of test flights have already been conducted with jatropha or Camelina biofuel blended with conventional kerosene
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