Abstract
A greenhouse experiment was conducted to study bio-activation of rock phosphate vis-a-vis seed treatment with phosphorus solubilzing microbes in enhancing P nutrition in cowpea and ragi. Bacillus megaterium and Aspergillus awamori were used to bio-activate Udaipur rock phosphate URP and seed treatment. In both the crops higher concentration of chlorophyll was noticed when inoculated through bio-activated rock phosphate with A. awamori . In cowpea, more number of nodules was observed in plants inoculated through bio-activated rock phosphate with A. awamori . Inoculation through bio-activated rock phosphate with B. megaterium contained higher total P content and total biomass in cowpea. In ragi, total P content and total biomass were higher in plants inoculated through bioactivation of rock phosphate with A. awamori . The results indicate that inoculation of phosphorus solubilzing microbes through bio-activation of rock phosphate enhanced the phosphorus uptake and growth of cowpea and ragi.
Highlights
Phosphorus (P) is an important plant nutrient required in large quantities
Goenadi et al (2000) used culture filtrates of Aspergillus niger to bioactivate Mussorie rock phosphate (MRP). These findings suggested that mixing the growth media of A. niger with MRP caused remarkable increase in citrate soluble P after incubation in laboratory
A green house experiment was conducted to evaluate differences were observed among bio-activation and the bio-activation of rock phosphate with phosphorus solubilizing microbes (PSMs) in seed treatment on chlorophyll content
Summary
Phosphorus (P) is an important plant nutrient required in large quantities. It is associated with several vital functions such as photosynthesis, cell division, cell organization and transfer of heredity. Water soluble phosphatic fertilizers are generally fixed by iron and aluminum oxides or hydroxides in acid soils and unavailable to plants These situations necessitate searching alternative sources which are reliable and cost effective. Recent studies indicated that bio-activation of rock phosphate with culture filtrates of phosphorus solubilzing microbes before application increases P availability soon after application and may sustain it later, since these organisms have assured supply of energy substrates. The application of bioactivated MRP with homogenized media and culture filtrate of PSMs as well as inoculation of soil with AM fungus Glomus ageratum enhances P uptake and growth of Leucaena and Sesbaenia (Hema, 2005). The present study was carried out to determine better method of application of PSMs by taking cowpea and ragi as a test plants
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