Abstract
The gfp gene, encoding the green fluorescent protein, was combined with the gusA gene, coding for the β-glucuronidase enzyme, in mini-Tn 5 transposon derivatives for use in Gram-negative bacteria. These mini-Tn 5 elements allow simultaneously monitoring of gene expression and localization of the marked bacteria. Introduction of the resultant mini-Tn 5 transposons into Rhizobium etli, Azospirillum brasilense and Pseudomonas stutzeri allowed us to visualise the interaction of these bacteria with their host plant. The dual-marker mini-Tn 5 transposons constitute a powerful new tool for studying gene expression and ecology of bacteria in the environment and during the interaction with plants.
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