Abstract
We investigated the antimicrobial activity of benzyl alcohol galactoside (BzO-gal), which was synthesized from benzyl alcohol (BzOH) using β-galactosidase (β-gal). Nowadays BzOH is used as excipients in the food, cosmetic, and pharmaceutical industries as fragrant and/or preservative. Based on our previous studies, we have been interested in BzO-gal as a safer preservative (as an alternative to BzOH). As a first step, the antimicrobial activity of BzO-gal against E. coli ATCC 25922 was examined. The culture media containing a white sauce (J&J Food Co.) were prepared, and thereafter BzOH or BzO-gal were also added to these media preparation. After E. coli cells grew up in these media, their viabilities were checked throughout the culture broth, followed by streaking them on the agar plate. Finally, it was observed that the antimicrobial characteristics 1% BzO-gal and 1% BzOH against E. coli were the same activities. However, 0.5% BzO-gal did not show antimicrobial activity, although 0.5% BzOH showed antimicrobial activity. Probably there was a loss of antimicrobial activity by transgalactosylation of BzOH. Nevertheless, we found the feasibility of the utilization of BzO-gal as an alternative to BzOH, as a safer preservative. It is expected that the use of BzO-gal will be extended to the food, cosmetic, and pharmaceutical industries.
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