Abstract
The aim of this work is preparation and use of an electrochemical DNA-based biosensor with a textured surface for the investigation of dsDNA host-guest interactions with specific chemicals in a biological sample of complex matrix such as urine. Results obtained at the search and optimization of polymer protective films over the DNA recognition layer at a glassy carbon electrode show that a polyvinyl alcohol membrane is the effective barrier able to stabilize the biosensor response in urine. The detection of acridines using the square-wave voltammetric (SWV) scan in the cathodic direction has allowed overcoming a problem of the electrochemical interference of DNA bases and acridines at the direct anodic scan. Height of the well-separated SWV peak at around 0.00V to +0.10V (which corresponds to the reduction of the oxidation products of both of acridines) depends on the concentration of acridines. The peak potentials of acridines were shifted by about 0.10V to the negative potential values in the urine medium compared to phosphate buffer. Intercalation of acridine yellow and proflavine into dsDNA was confirmed both in phosphate buffer and in urine.
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