Behavioral and Physiological Reactions to a Sudden Novel Object in the Weanling Horse: Quantitative Phenotypes for Future GWAS

35 Refined phenotyping methods for the equine cardiac startle response

Objective: Poor physiological regulation in response to threat is linked to multiple negative developmental outcomes including anxiety, which is highly prevalent and impairing in young children with neurodevelopmental disabilities like fragile X syndrome (FXS) and autism spectrum disorder (ASD). The present study contrasted cardiac startle response in pre-school-aged children with FXS, with and without ASD, to children with non-syndromic ASD (nsASD) and neurotypical controls (NT). The relationship of cardiac startle to non-verbal mental age (NVMA), ASD severity, and parent-reported anxiety was also examined.Method: Four age-matched groups of pre-school children participated including those with FXS without ASD (FXS-Only, n = 21), FXS with ASD (FXS+ASD, n = 17), nsASD (n = 42), and NT children (n = 27). Participants viewed a silent movie during which a single 200 ms 98-decibel white noise burst occurred. Cardiac activity was analyzed for pre-stimulus respiratory sinus arrhythmia (RSA) and the inter-beat intervals (IBI) at the auditory stimulus and 10 s post-stimulus. The Spence Pre-school Anxiety Scale, Autism Diagnostic Observation Schedule-2nd Edition, and Mullen Scales of Early Learning were examined in relation to startle response.Results: The nsASD group demonstrated heightened cardiac activity at the auditory stimulus and 10 s post-stimulus compared to the NT controls. Neither of the FXS groups showed differences from any other group. Higher pre-stimulus RSA was associated with reduced cardiac response across groups, while the relationship between cognitive ability and ASD severity to cardiac response varied between groups. Parent-reported anxiety was not associated with cardiac response for any group.Conclusion: These findings demonstrate group distinctions in cardiac responses to auditory startle. Although FXS and ASD share behavioral characteristics, the nsASD group showed a heightened cardiac startle response compared to the NT group that was not present in the FXS groups with or without ASD. Non-verbal mental age was associated with greater stimulus or post-stimulus reactivity for all groups except the FXS+ASD group, which showed no association between startle response and any clinical outcomes. Increased understanding of the relationship between physiological regulation and clinical outcomes will assist in identifying the timing and targets for effective interventions for individuals with neurodevelopmental disabilities.

While over 40 neonatal pain assessment scales have been published, owing to a lack of consensus and standardized metrics, there are more than 100 assessment indicators with varying descriptors and quality differences. This study aims to reach a consensus on optimal and comprehensive variables for neonatal pain assessment, leading to the development of a multidimensional neonatal pain response variable set. This study consisted of three phases: (1) A literature review was conducted to identify influencing factors and assessment indicators of neonatal pain response. (2) Panel meetings involving neonatal healthcare professionals evaluated and screened factors and indicators to develop an initial draft of the variable set. (3) Through two rounds of Delphi study achieved consensus, and determined the neonatal pain response variable set. Through a literature review and a panel meeting, the identified factors and indicators were categorized into contextual, physiological, and behavioral variables, forming an initial draft of the variable set. Sixteen professionals participated in two rounds of the Delphi study, with response rates exceeding 70%, and authority coefficients surpassing 0.7 in both rounds. The final iteration of the variable set includes 9 contextual variables, 2 physiological variables, and 5 behavioral variables. Conclusion: Neonatal pain response variable set developed in this study is scientific, comprehensive, and multidimensional, aligning with the characteristics of neonatal pain response and clinically applicable. The inclusion of contextual variables enhances the ability to confront the complexity of clinical environments and individual differences. It can provide a practical and theoretical basis for clinical research on neonatal pain assessment.What is Known:• Neonatal pain assessment relies on scales used by healthcare professionals currently. But there is no “gold standard” for neonatal pain assessment.• While over 40 neonatal pain assessment scales have been published, owing to a lack of consensus and standardized metrics, there are more than 100 assessment indicators with varying descriptors and quality differences. Most of scales overlook the clinical environment complexity individual differences in pain responses, diminishing the accuracy and applicability.What is New:• In addition to the commonly used physiological and behavioral variables in the scales, we have incorporated contextual variables to better address the complexity of clinical environments and individual differences in pain responses.• Through an evidence-based approach, developed a neonatal pain response variable set comprising 9 contextual variables, 2 physiological variables, and 5 behavioral variables.

Cardiac Response Dynamics in Newly Diagnosed Light-Chain Amyloidosis Patients With Early and High-Quality Hematologic Response

College students have low levels of physical activity (PA); however, PA enhancement is suggested to serve as a gateway to the improvement of other forms of health behavior. Graduate Ready for Activity Daily (GRAD) by Sallis et al. (1999) is a PA enhancement course for college students immediately before graduation. The program contains the behavior change skills needed to adopt and maintain regular PA. Moreover, there is also a need to consider PA intervention for freshmen, from the same viewpoint as GRAD. The purpose of this study was to determine whether or not a behavioral science-based physical education program with homework would have positive effects on the psychological, behavioral, and physiological variables related to the PA of college freshmen. The project was named “First-Year Physical Education” (FYPE).College freshmen from an institute of technology in the Kinki area of Japan participated in this study (N=993; intervention group, N=497; non-intervention group, N=496). The programs that were common to all the classes were as follows (the numbers correspond to the sequence of activities in the program): (1) guidance, (2) health-related physical fitness test, (3)–(6) sports activity, (7) lecture on PA and health, (8)–(12) sports activity, (13) health-related physical fitness test, and (14) summary of the program. The PA enhancement programs were meant only for the intervention group. The programs consisted of education on behavioral change skills (decisional-balance analysis, changing self-talk, relapse prevention, social support, shaping, and so on), and out-of-class practical assignments such as active homework (self-monitoring and goal setting with regard to PA). The duration of the weekly program was 3.5 months. We measured psychological variables (self-efficacy and decisional balance [pros–cons] for exercise), behavioral variables (PA level according to the intensity and frequency of categorized PA), and physiological variables (health-related physical fitness, i.e., cardiovascular endurance, flexibility, muscular endurance, and percentage body fat). These variables were measured both before and after the class term; the intervention and the non-intervention groups were subsequently compared.Two-way ANOVA and post hoc test revealed a significant intervention effect for psychological variables such as self-efficacy and the pros of exercise. With regard to the behavioral variables, a significant intervention effect was observed for both the PA levels of “exercise and sports” and “daily activity” and the frequency of the categorized PA (daily PA, health-related exercise such as aerobic exercise, stretching, and muscular exercise). In the health-related physical fitness test with regard to physiological variables, a significant intervention effect was observed for muscular endurance. These results suggest that this behavioral science-based physical education class with homework has comprehensive positive effects on the psychological, behavioral, and physiological variables related to the PA of college freshmen.

The use of non-validated pain measurement tools to assess infant pain represents a serious iatrogenic threat to the developing neonatal nervous system. One partial explanation for this practice may be the contradictory empirical data from studies that use newborn pain management tools constructed for infants of different developmental stages or exposed to different environmental stressors. The purpose of this review is to evaluate the evidence regarding the physiologic and behavioral variables that accurately assess and measure acute pain response in infants. A literature search was conducted using PUBMED and CINAHL and the search terms infant, neonate/neonatal, newborn, pain, assessment, and measurement to identify peer-reviewed studies that examined the validity and reliability of behavioral and physiological variables used for investigation of infant pain. Ten articles were identified for critical review. Strong evidence supports the use of the behavioral variables of facial expressions and body movements and the physiologic variables of heart rate and oxygen saturation to assess acute pain in infants. It is incumbent upon researchers and clinical nurses to ensure the validity, reliability, and feasibility of pain measures, so that the outcomes of their investigations and interventions will be developmentally appropriate and effective pain management therapies.

A number of studies have demonstrated that physiological and behavioral cardiovascular disease (CVD) risk factors aggregate within families. This fact, and the potential mediating role that the family plays in behavior change, have led to the development of family-based CVD risk reduction programs, including the San Diego Family Health Project. The aggregation of behavioral, physiological, and cognitive changes within families was assessed during a 1-year intervention. We found evidence of modest but significant aggregation of change. There was more aggregation of change in behavioral variables than in physiological or cognitive variables. More significant correlations were found among 3-day food record measures than among 24-hour recall dietary measures, suggesting an influence of assessment method. Aggregation of change within families was stronger within generations than across generations. These data point to the importance of involving all age groups in health promotion programs.

Cardiac and forearm plethysmographic responses to high intensity auditory stimulation

Author response: Early-life experience reorganizes neuromodulatory regulation of stage-specific behavioral responses and individuality dimensions during development

Decision letter: Early-life experience reorganizes neuromodulatory regulation of stage-specific behavioral responses and individuality dimensions during development

Leaf-cutting ants exhibit an aggressive alarm response. Yet in most alarm reactions, not all of the ants encountering a disturbance will respond. This variability in behaviour was investigated using field colonies of Atta capiguara, a grass-cutting species. Crushed ant heads were applied near foraging trails to stimulate alarm reactions. We found that minor workers were disproportionately likely to respond. Only 34.7±2.8% of ants travelling along foraging trails were minor workers, but 82.1±6.1% of ants that responded were minors. Workers transporting grass did not respond at all. The alarm response was strongest at the position and time where minors were most abundant. Ants were more likely to respond when they were travelling along trails with low rather than high traffic. Minor workers followed a meandering route along the trail, compared with the direct route taken by foragers. We argue that an important function of minor workers on foraging trails is to patrol the trail area for threats, and that they then play the key role in the alarm reaction.

In selecting rats for behavior, we observe a direct natural effect and affect the nonspecific stress function. In this process, new behavioral phenotypes appear in the strain under selection. They differ from the selected forms in the selection criterion. In the GC strain, a large proportion of the so-called nervous rats emerge. The criterion presumes the selection for the long cataleptic freezing character, whereas the nervous rats display elevated motor excitement: running, jumping, and vocalization. The main purpose of our study was to assess phenotypic indices in GC rats (abbreviated from genetic and catatonia) and recognize principal components of variability for emotional and weight indices. Rats of the ancestral Wistar population were taken as control. The following indices were measured: time of cataleptic freezing, excitement level, blood pressure, acoustic startle response, seizure activity, and weights of the heart, kidneys, adrenals, and spleen. Multivariate analysis methods were applied: factor analysis and principal component analysis. We confirmed the inclination of GC rats of the generation studied to freezing in quiet surrounding and after a strong acoustic sti - mulus. More pronounced startle responses, moderate hypertension, and larger weights of the heart and adrenals were noted. Two principal variability components were recognized: startle amplitude (PC1) and morphofunctional variability (PC2). The figure shows different locations of Wistar and GC individuals in principal component coordinates. The principal component method confirmed the genetic relationship between the startle and nervousness responses. It was shown that in PC2 the indices of heart, kidney, adrenal, and spleen weight exert negative effects, whereas the effects of startle and nervousness were positive. In the same component, an increase in the startle and nervousness responses positively correlates with the relative weights of the heart and adrenals. Differences in the directions of the contributions to the second component of morphofunctional variability are discussed.

Individual wellness comprises both psychological and physiological wellbeing, which are interrelated. In long-term monitoring of wellness, both components should be included. Work-related stress and burnout are persistent problems in industrial countries. Early identification of work-related stress symptoms and early intervention could reduce individual suffering and improve the working productivity and creativity. The goal of this study was to explore the relationship between physiological and psychological variables measured at home by the users themselves or automatically. In all, 17 (3 males and 14 females, age 40-62) people participating in a work ability rehabilitation program (due to work overload) were monitored for three months. Physiological and behavioral variables (activity, bed occupancy, heart rate (HR) and respiration during night, HR during day, blood pressure, steps, weight, room illumination, and temperature) were measured with different unobtrusive wireless sensors. Daily self-assessment of stress, mood, and behaviors (exercise, sleep) were collected using a mobile phone diary. The daily self-assessment of stress and the Derogatis stress profile questionnaire were used as reference for stress status. Results show modest, but significant pooled overall correlations between self-assessed stress level, and physiological and behavioral variables (e.g., sleep length measured with wrist-worn activity monitor: rho = -0.22, p < 0.001, and variance of nightly bedroom illumination: rho = 0.13, p < 0.001). Strong, but sometimes conflicting correlations can be found at individual level, suggesting individual reactions to stress in daily life.

Acoustic recordings were used to investigate the cardiac responses of a captive dolphin (Tursiops truncatus) to sound playback stimuli. A suction-cup hydrophone placed on the ventral midline of the dolphin produced a continuous heartbeat signal while the dolphin was submerged. Heartbeats were timed by applying a matched-filter to the phonocardiogram. Significant heart rate accelerations were observed in response to playback stimuli involving conspecific vocalizations compared with baseline rates or tank noise playbacks. This method documents that objective psychophysiological measures can be obtained for physically unrestrained cetaceans. In addition, the results are the 1st to show cardiac responses to acoustic stimuli from a cetacean at depth. Preliminary evidence suggests that the cardiac response patterns of dolphins are consistent with the physiological defense and startle responses in terrestrial mammals and birds.

The issue of materialism has recently been raised again. Mr. Putnam argues against philosophical behaviorism [4]. Such a position holds, as he construes it, that statements like ‘Jones is angry’ can be analyzed in solely behavioral terms. When one argues against philosophical behaviorism, he might be expected to distinguish this metaphysical position from behavior science. Putnam, however, does not make the distinction. Consequently he argues against both. I shall first state the distinction between these two different things, namely, philosophical behaviorism and behavior science, as I see it. The behavior scientist adopts the thesis that in principle it is possible to predict future behavior on the basis of data concerning environmental, behavioral, and physiological variables. All three of these he considers in physical terms. The behavior scientist thus speaks about physical objects and properties of such. Talking in such terms, he believes that it is in principle possible to coordinate to statements asserting that person X has or is in state of mind Y another statement, employing only the above mentioned physical terms, such that either both are true or both are false. The reasons for the behavior scientist's program are the well known quandaries involved in the observation of other people's minds and the need for intersubjective verification in science. One can further distinguish between a narrower and a broader view of behavior science. The former restricts itself to environmental and behavioral variables at what some call the macro level; the latter includes, or even concentrates upon, physiological variables. As scientists neither the behaviorist nor the physiologist asks or answers philosophical questions, either epistemological or ontological, about minds, bodies, and mental contents.