Abstract
A beam-scanning approach is demonstrated to improve signal-to-noise ratio and to reduce photo-induced sample damages in nonlinear hyperspectral imaging, such as broadband coherent anti-Stokes Raman scattering (BCARS) microscopy. Signals generated on a fast scanning axis are transferred to the slit of a monochromator, then onto the vertical axis of a CCD. This configuration acquires the whole BCARS spectra on the fast scanning axis at once, reducing the overall data transfer time. The fast scanning speed by a resonant beam scanner at 5 kHz greatly reduces sample damage from accumulative photo excitation, allowing for a higher excitation laser power to generate a stronger nonlinear signal. The optical characteristics related with this configuration and parameters are discussed and a few examples that demonstrate the advantage of the beam-scanning method for BCARS tissue imaging are presented.
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