Abstract
In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
Highlights
These days are characterized by rapid population growth, international trade and globalization, and for this reason the risk of influenza pandemics looms larger and larger [1]
We suggest a method based on the isolation of quantum dots (QDs)-labeled HA by glycan-modified magnetic nanoparticles (MPs) and on electrochemical detection of the MPs-HA-glycan-QDs complex, which is formed due to the specific and selective binding between glycans and HA
In our previous study we described a MPs-based isolation method for standard viral proteins labeled with CdS [26]
Summary
These days are characterized by rapid population growth, international trade and globalization, and for this reason the risk of influenza pandemics looms larger and larger [1]. Influenza is one of the most frequently occurring respiratory diseases, which causes approximately 500,000 deaths every year. The influenza virus is subject to genetic mutations, mainly due to the lack of proof-reading activity of its Molecules 2013, 18 polymerase [2]. Influenza infections mostly occur as seasonal epidemics or less frequently, as influenza pandemics. During twentieth century there were three, with shortening delays between them: Spanish flu in 1918, Asian flu in 1957 and Hong-Kong flu in 1968. The twenty first century has been marked by a few cases of pandemics (swine and avian influenza) with a small number of victims, but with much larger economic impact
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